Principle of the photomultiplier tube:
The necessary steps of the quantitative determinations methodology are,
- Forming an absorbing species
- Selection of the measurement wavelength
- Controlling factors influencing absorbance
- Validation of Beer and Lambert's law
A photomultiplier tube is based on the principle of successive rise in the number of electrons. The output signal through photoemission from a series of electrodes known as dynodes is much larger than the original signal.