Water regain capacity:

Getting back to water regain capacity, it is commonly expressed as the amount of water (in mL) imbibed through one gram of dry xerogel on swelling. It does not include the interstitial liquid among the grains. In that case of dextran and polyacrylamide gels, it has been observed that there is a close relationship between the water regain and the fractionation features. If the water regain is low, it is expected in which the fractionation range is at a low molecular mass. In that case of agrose gel, it is percentage of gel matrix within the gel grain is taken alter of the water regain. Although working with nonaqueous solvents, the analogy is taken with water regain. Ultimately, the particle size of the gel grain is a significant variable. It affects the degree of zone broadening, the resolution, the dilution and the flow rate. Since a matter of fact, the above declared information should be available to the user before a particular gel is put to use for chromatographic work.