Synthesis:

Triacylglycerols are synthesized from fatty acyl CoAs and glycerol 3-phosphate in another figure.  The   glycolytic   intermediate   dihydroxyacetone phosphate is first decrease to glycerol 3-phosphate that is, in turn, acylated through glycerol-3- phosphate acyltransferase to create lysophosphatidic acid. This is then reacted with an additional acyl CoA molecule to form phosphatidic acid.  Removal of the phosphate set from phosphatidic  acid produces diacylglycerol (DAG) that is additional acylated with a third acyl CoA molecule to form triacylglycerol. ATP is not included in the biosynthesis of triacylglycerols. Alternatively the reactions are driven through the cleavage of the high-energy thioester bond among the acyl moiety and CoA. Both phosphatidic acid (phosphatidate) and DAG is also used as a second messenger in cell signaling and DAG is also used in the synthesis of membrane phospholipids.