Modification of tRNA:
Transfer RNA molecules are notable for containing generally nucleotides shown in the figure such as 1-methylguanosine (m1G), pseudouridine (Ψ), dihydrouridine (D), inosine (I) and 4-thiouridine (S4U). These are created through modification of guanosine and uridine after tRNA synthesis. For instance, inosine is generated by deamination of guanosine.
Figure: Some modified nucleosides found in tRNA molecules.