High-Performance Thin Layer Chromatography (HPTLC):

In order to have better resolution and sensitivity in TLC, it could be coupled along with mass spectrometry (MS), high- performance liquid chromatography (HPLC), Fourier- transform infrared (FTIR) etc. Since already elaborates in Sec, TLC could be compared along with liquid-liquid chromatography whereas solute is detected in the presence of the mobile phase that could be so chosen in which it might not interfere with the detection wavelength. Reverse phase version of TLC has been proposed where better separation can be obtained than that with normal TLC. Thus, the performance of TLC can be improved by improving the quality of adsorbent and sample application procedure done by choosing smaller particle size, decreasing the plate length and through using smaller quantity of sample as elaborates below.

Quality of Adsorbent:  Specially purified silica gel with average particle diameter of 3-5 µm is used and it may also be modified with chemically bonded layers. Such an adsorbent may have 5000 theoretical plates providing a much improved performance over the conventional TLC. Therefore, a better separation might be achieved within much shorter time.