Activation energy and transition state

The energy changes which take place during the course of a particular biochemical reaction are described in Figure In all reactions there is an energy barrier which has to be overcome  in order for the reaction  to proceed.  This is the energy need to transform the substrate molecules into the transition state – an unstable chemical form category-way among the substrates and the products. In the transition state it has  the  highest  free  energy  of  any  part  in  the  reaction  pathway.  Gibbs free energy of the activation (ΔG‡) is equivalent to the variations in free energy among the transition state and the substrate in figure. An enzyme works through stabilizing the transition state of a chemical reaction and decreasing ΔG‡ .the enzyme does not alter the energy stages of the substrates or the products. Therefore an enzyme increases the rate at that the reaction occurs but has no effect on the whole change in energy of the reaction.