Liquid coated packing:

There are four various methods now in use for preparing liquid coated packing for liquid-liquid column chromatography:

1. Solvent evaporation method.

2. In situ coating process.

3. The solvent filtration method.

4. The equilibration method.

The all four supports for liquid-liquid partition chromatography

Within liquid-liquid partition chromatography, a stationary liquid phase is supported on an inert support. An ideal support material has to meet the subsequent requirements.

• It should be chemically inert and must not dissolve or swell in the stationary phase.
• It should show good wetability through the stationary phase and it should neither dissolve nor react along with the mobile phase.
• It should consist of particles as identical as probable that permit the most uniform and reproducible packing.
• It should have huge sufficient surface to retain the stationary phase as a thin uniform film. Porous supports commonly meet these needs.
• It should permit the columns to have an acceptable pressure drop as regards the mobile phase.
• It should have enough mechanical stability. It must not grind during column packing, impregnation of stationary phase or regeneration of support material.
• While applied for routine analysis or for preparative needs, it must be relatively cheap, simply available and permit regeneration.