Applications of Fluorimetry and Phosphorimetry:
You have studied about the fluorescence and phosphorescence spectroscopies. You have learnt that these techniques can be categorised as the examples of molecular emission spectroscopy. Here you have also learnt about the difference among fluorescence and the phosphorescence phenomena and how transition time makes a radical difference in the utility of these two methods. Phosphorimetry lags behind fluorescence as an analytical tool because of lack of suitable instruments. Therefore, with advent of time, greater strides have been made in the field of instrumentation and in the last two decades great progress has been made in the field of phosphorescence spectroscopy. The newer methods have shown that measurements at room temperature instead of at low temperature can widen the domain of analytical phosphorimetry.
In this unit, you would learn about important applications of fluorescence and phosphorescence spectrometry in quantitative and qualitative analysis of a variety of inorganic as well as organic compounds. Further, we would take up some specific applications in the areas of environmental and biochemical analysis. You would learn how we can effectively go down to the concentrations in the range of nanogram to picogram (ng-pg) of the substances to be analysed by these methods.
Objectives
After studying this unit, you will be able to:
- List the applications of phosphorescence and fluorescence measurements in chemical analysis,
- Elaborates the analysis of gaseous pollutants through chemiluminescence,
- Define the analysis of water pollutants through fluorimetric methods,
- State the principle behind an analysis of biological samples using photoluminescence methods,
- Provides examples of the organic complexing ligands producing fluorescence along with metals,
- Enumerate the kinds of reactions producing fluorescence,
- List factors responsible for fewer analytical applications of phosphorimetry, and
- Elaborates the importance of room temperature phosphorescence