Precautions:

As fluorimetry is an extremely sensitive method, the following precautions should be followed without exception although handling the cuvettes.

• The optical surfaces of the cell should not be touched along with hand as it invariably leaves an invisible film which may change the light transmission and reflection features of the cell, especially in the ultraviolet region.
• The cuvettes should be handled only at the top portions of the side plates in which do not face the optical axis.
• The samples should be transferred to the cuvettes along with the help of a dropper or a pipette.
• The cuvettes should be rinsed within the analyte solution before filling and the overfilling should be prevented.