Average number of moles Assignment Help

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Average number of moles:

This fraction is equivalent to average number of moles of a solute in the mobile phase at any instant divided by the total number of moles of the solute in the column.

v = u ×   Moles of the solute in mobile phase/ Total moles in the column

A number of moles of the solute in the mobile and stationary phases is the product of their concentration and respective volumes

v = u × C M VM/(C M VM   + C S VS)

where, C and V denote the concentration and volume, correspondingly

v = u × 1/(1 + C S VS /C M VM)

We know that K =  C S/C M

Thus, we have a relation between solute migration and distribution constant

v = u × 1/(1 + KVS /VM)

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