Liquid-liquid Chromatography:

Liquid-liquid chromatography is sometimes called liquid partition chromatography. It is based on the separation of the solutes through their differential partitioning between two immiscible phases. This usually involves a stationary phase coated on an inert solid support, normally silica gel and an immiscible mobile phase. Most generally the stationary phase is more polar than the mobile phase. In some circumstances, therefore, it is advantageous to reverse the roles so that the stationary phase is less polar. This variation is called as reversed phase partition chromatography. The procedure of liquid-liquid chromatography is similar to simple batch extraction between two immiscible liquids in a separatory funnel. A successive series of such extractions forms the basis of countercurrent distribution, which is more efficient than simple one stage extraction.  However,   liquid-liquid chromatography is many times faster and more efficient than countercurrent extraction. This is the result of the large interface between moving and stationary phases.