Selection of a protein source

Before attempting to purify a protein, the first and main thing to consider is the source of beginning material.  Proteins differ in their tissue and cellular distribution therefore if a protein is identified to be abundant in one particular tissue example is. Kidney it makes sense to begin the purification from this source.  Also,  many  sources  are more  readily  available  than others  and this should  be taken  into account  too. Todays, with the use of recombinant DNA methods, even scarce proteins can be expressed synthesized in eukaryotic cells or bacteria and relatively huge amounts of the protein subsequently obtained.