Requirements of an appropriate mobile phase:

The requirements of an appropriate mobile phase are given as under:

i) It should have viscosity as low as possible for higher column permeability and or efficiency.

ii) It should give a retention/ capacity factor for solutes preferably between 2 and 10.

iii) The cost, toxicity, purity and stability should be given due consideration.

iv) A detector response to the solutes should not be interfered with.