Experimental Set Up:

At this point, with the background that we already have, it may be important to discuss the experimental set up of liquid column chromatography. Conventionally, in the classical set up there is a simple column, packed with an adsorbent or a support material coated along with a stationary phase. A mixture is fed to the column that is then irrigated along with the mobile phase. An eluant is collected within small increments and put to analysis. Therefore, with time, various improvements have taken place.

In classical liquid chromatography, the column is used just once and is then discarded. Therefore, the packing in a column has to be refilled for each separation and this amounts to a significant expense of both manpower and material. In classical liquid chromatography, a sample application needs a few skill and time on the part of the operator. The Solvent flow is achieved through gravity feeding of the column. Separations require several hours. A detection and quantitation are completed through the manual analysis of individual fractions.