Liquid chromatography:

While concluding the discussion on liquid chromatography, it will be important to point out that the most significant advancement in liquid chromatography came in the form of high performance or high pressure liquid chromatography. This improved version is more or less available for different forms of liquid chromatography. The separation is performed on a column under pressure. The column usually contains particles of very small size, 3 -10 µ m. The material may be an adsorbent or materials which allow partition, ion exchange or molecular permeation. The pressure commonly used is around 6000 psi. A mobile phase might be composed of one solvent or a mixture of solvents with or without modifiers. Now high pressure thin layer chromatography (HPTLC) has also gained prominence.