Sample size:

The sample volume might vary depending upon the amount and rate of evaporation of the sample within the sample port in the case of liquids/ solutions of solids or on the pressure of gas/ vapor sample holed within the sample container. In the case of gases, it is a general practice to vary the amount of sample through introducing various volumes at a constant pressure. The sample size will obviously be associated to the kind of solute-stationary phase interaction (linear or non-linear) and consequently to the shape of the final chromatographic peak acquired.