Example:

0.32 g stainless steel sample was dissolved in nitric acid and the resulting solution was made to 100 cm³ with water. There are five standards and the sample solution was aspirated within flame for the determination of nickel. The subsequent observations were made.

Concentration of Nickel (ppm)                            Absorbance

2                                                                                  0.126

4                                                                                  0.250

6                                                                                  0.374

8                                                                                  0.500

10                                                                                0.626

Sample                                                                       0.226

Calculate the percentage of nickel in steel sample.

Solution

Let us prepare the calibration plot by taking the concentration on X-axis and absorbance on Y-axis, as display as follows According to the calibration plot the sample concentration is found to be 3.612 ppm. That is corresponds to 1.12%.