Applications:
Thin layer chromatography is widely used for qualitative analysis; almost any mixture can be partially resolved. The Inorganic separation of metals within alloys, soils and geological samples and so also organic compounds created during synthesis work or the analysis of natural products could be simply achieved through TLC. It is ideally suited for following the course of complex reactions, quality control, purity checks, plant extracts, biochemical preparations, clinical diagnosis, forensic tests and many more. It is primarily used for qualitative identification by comparison of the Rf values with those of standards run under identical conditions or by removing the material from the chromatogram and subjecting it to further tests by other techniques. The versatility of this technique has resulted in a rapid spread of its use in all the fields. Particularly sharp separations have been acquired for vitamins, fatty acids, amino acid s, lipids in serum, dyes and glycerides, sugars, pesticides, etc.