Theory of chromatography:
This particular unit elaborates the both theoretical and practical aspects of gas chromatography. A theory of chromatography has been elaborates within Unit 4 whereas there was an emphasis on gas as a mobile phase. Here, we would recapitulate the basic concepts with a total focus on gas chromatography. A few of the symbols used here might be different from those used in Unit 4 but they have been properly explained to prevent any confusion. Within the basic aspects, we will discuss the working of a gas chromatograph and the column and solvent efficiency. This is followed through a discussion on instrumentation involve requirements of a carrier gas. This is followed through a discussion on columns and stationary phase support and liquid phases and at last the detectors. The next section defines sampling and sample injection. The final section of the unit illustrates some representative applications in terms of both qualitative and quantitative analysis.
Objectives
After studying this Unit, you should be able to
- Define the basic aspects of gas chromatography particularly in terms of column and solvent efficiency,
- Elaborates the importance of characteristics of carrier gas,
- Define about different types of columns,
- Choose appropriate stationary phase support and liquid phase,
- Elaborates the working of different types of detectors and select the appropriate one,
- Describe the importance of sampling and sample injection in GC unit, and
- Define the utility of the method for both qualitative and quantitative analysis.