Fluorescence quenching:

The fluorescence quenching might be defined as the decrease in fluorescence intensity of a molecule as a consequence of the interaction of the excited state of the molecule along with its surroundings.

Self quenching includes the collisions among excited and ground state molecules leading to an increase in the amount of radiation less relaxation.

The following precautions should be observed although handling the cuvettes during fluorescence measurement.

• An optical surfaces of the cell should not be touched along with hand.
• A cuvettes should be handled only at the top portions of the side plates which do not face the optical axis.
• A samples should be transferred to the cuvettes along with the help of a dropper or a pipette.
• A cuvettes should be rinsed along with the analyte solution before filling and these should not be overfilled.