Determination of blood glucose Assignment Help

Assignment Help: >> Fluorimetric Determination of Blood Glucose - Determination of blood glucose

Determination of blood glucose:

The determination of blood glucose is based on the subsequent reactions.

Step 1: A enzyme glucose oxidase catalyses the oxidation of β-D-glucose to form D-glucono-1,5-lactone  and hydrogen peroxide.

1895_Determination of blood glucose.png

Step 2:  D-glucono-1, 5-lactone after that spontaneously gets hydrolysed to produce gluconic acid.

D-Glucono-1,5 -lactone  +H2O → D-Gluconic acid

As the α -D-glucose is rapidly converted to the beta form, thus, all of the glucose is measured at a single time. This glucose oxidase catalysed reaction could be monitored through fluorescent detection of the consumption of oxygen or through monitoring the production of hydrogen peroxide. An amount of hydrogen peroxide generates is determined through using a fluorophore named luminol.

 

425_Determination of blood glucose1.png

1628_Determination of blood glucose2.png

The detection limit of glucose through this method is 50 µg/ml. This reaction could be used for analysis of fructose or sucrose also. Presently some biosensors are also being established to measure blood glucose stages.  These biosensors are based on sensitive fluorescence measurements that work through monitoring changes within the intrinsic FAD (flavin adenine dinucleotide) fluorescence of glucose oxidase. FAD is the cofactor of the enzyme.

Analysis of Blood Serum Analysis of Calcium Ion
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd