Extractive Fluorimetry:

In several fluorimetric determinations the interferences are too serious to be ignored. These interfering species required to be removed before taking up the determination. The ion to be determined is extracted out of the sample and leaving the interferences behind. Solvent extraction is one such method in which facilitates the removal of interferences e.g. Tl (III) is first extracted in benzene along with crystal violet that in turn is substituted through butyl Rhodamine B. Such as extractive photometry that is so extensively used in analytical chemistry, extractive fluorimetry is getting extremely popular e.g. Bi(III) is analysed along with dibenzoylmethane  at 1µ g level or niobium (Nb) with oxine could be determined at 0.18 ppb level.  Beryllium could be analysed through dibenzoylmethane along with pyridine and as small as 0.0004 µ g/ml of Be could be conveniently analysed through extractive fluorimetry.

Having learnt about different kinds of reactions in which aid within the fluorimetric determination we could now take up the applications of fluorimetric determinations of inorganic substances. Within a broad way, the applications of fluorescence within the analysis of inorganic species have been grouped under three heads as given below.

• Inorganic substances showing luminescence
• Fluorescence with inorganic reagents
• Fluorescence with organic reagents

The first group pertains to direct analysis while the other two exploit indirect methods. Let us starts along with inorganic substances having intrinsic fluorescence that is they are fluorescent in nature.