Quantitative applications:

Additionally, the life time measurements in fluorescence are extremely useful for the determination of the analyte. A fluorimetric method are preferred over the spectrophotometric methods if the concentration of the material to be analysed is too small; as small as 0.1 ng of analyte within 10 ml solution could be conveniently analysed through fluorimetry. The selectivity of fluorimetry, therefore, is limited through the wide spectra without much finer details. In addition, the positions of bands are not sensitive to the molecular structural details. Thus, fluorimetry is not commonly useful for molecular identification.

Despite the fact that very few fluorescence species are in existence, the fluorimetric determinations have their own place.  Knowledge of excitation and emission wavelength of the analyte facilitates the quantitative analysis. Most of the organic compounds and metal chelates are usually analysed in the UV-Vis region of 200,800 nm with appropriate excitation wavelengths. Let us learn about the basic principle behind the quantitative applications and also the factors that affect such determinations.