Fluorimetric Determination of Blood Glucose:
As you are aware, the analysis of blood glucose is most critical for diagnosis of diabetic patients. A people along with diabetes mellitus requires to constantly monitor their blood glucose stages in sequence to detect and control fluctuations in glucose level that could lead to hyperglycemia (high blood glucose levels) or hypogl ycemia (low blood glucose levels). It is, thus, essential to have a specific, simple and rapid test for the concentration of glucose in the blood. Here again fluorescence method could be exploited.
Presently, blood glucose levels are measured through a procedure based upon the enzyme named glucose oxidase. Because an enzyme is used, it is specific for just D- glucose, and will not be subject to interferences from other molecules within the blood. The determination of blood glucose is based on the subsequent reactions.