Interfering species:

In a few fluorimetric determinations we might have some species, whose absorption band overlaps along with the absorption or emission bands of the analyte. Within such cases, these species affect the fluorescence emission intensity.

In present years, there has been a growing required for developing highly sensitive and selective probes for the detection of metal ions in environmental and biological samples. A variety of divalent metal ions are known to be involved in the structural, catalytic, and regulatory aspects of the biological system, and a few such metal ions serve as prognostics of certain human diseases. For instance, Cu²⁺, Zn²⁺, and Fe²⁺ have been found to be included in aggregating β-amyloid peptides during the onset of Alzheimer's disease. Further, analysis of different biological samples involving the body fluids, etc. is crucial for diagnostic purposes and to plan an appropriate treatment strategy. It, thus, becomes pertinent which we develop appropriate analytical methods for the analytical determination of the metal ions and other species within environmental and biological samples. Let us take up the role of fluorimetry in environmental monitoring.