Enzyme velocity

The rate of an enzyme-catalyzed reaction is frequently known its velocity.  The Enzyme velocities are generally reported as values at time zero initial velocity, symbol 1V₀; μmol min, because the rate is fastest at the point where no product is still present.   This  is,  as   the  substrate   concentration   is  greatest   before  any substrate  has been transformed  to product because enzymes may be subject to feedback  inhibition  through their  own  products  and/or because  with  a reversible reaction   the  products   will  fuel  the  reverse   reaction.  The  Experimentally   V₀ is measured   before  more  than  approximately   10 percent  of  the  substrate   has  been converted  to product in order to minimize  like complicating  factors. A classic plot of product formed against time for an enzyme-catalyzed reaction described an initial period of rapid product formation that gives the linear portion of the plot which is shown in figure. This is followed through a slowing down of the enzyme rate as substrate is used up and/or as the enzyme loses activity.  V₀    is acquired through drawing a straight line via the linear part of the curve beginning at the zero time-point. The slope of this single line is equal to V₀.