Resolution of species:

There could be two approaches to rise the resolution of species.

1. One component moves at a faster pace and the other at a slower rate.

2. The rate of zone broadening for the two species is decreased. The two situations are illustrated in Figure.

Figure: Two approaches to improve the binary separation; (i) initial chromatogram with overlapping peaks improved by; (ii) increase in band separation; and (iii) decrease in band width