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Leading and lagging strands:
The primary scheme of replication of double-stranded chromosomal DNA in eukaryotes follows which for bacterial DNA replication a lagging strand and leading strand a rare synthesized, the latter including discontinuous synthesis through Okazaki fragments. The RNA primers needs are made through DNA polymerase that carries a primase subunit. The DNA polymerase α initiates synthesis of the lagging strand that making first the RNA primer and then extending it with a small region of DNA. The DNA polymerase δ then synthesizes the rest of the Okazaki fragment. The leading strand is synthesized through DNA polymerase δ. The δ enzyme has 3' → 5' exonuclease activity and so can proof- read the DNA made but DNA polymerase α has no like activity.
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