Elution Analysis:

The elution analysis is the most common technique in chromatography. Employing elution analysis, complete separation can be achieved. This technique is being hugely used for quantitative analysis. An associatively inert solvent is used in large quantities to transport the components down the column.

The nature of the solvent influences the equilibrium between stationary and mobile phases. The nature of the solvent can also affect the shape of the distribution isotherms. Bands that might travel down the column with a symmetrical concentration profile (Figure (c)) with one solvent might be quite unsymmetrical with another. When elution analysis is used to separate materials with widely differing distribution coefficients, the use of single elution solvent is not practical. If the eluent chosen is strong enough to remove the most strongly held materials in a reasonable time, it will carry the more weakly held materials through the column too rapidly. Instead, if the eluent is weak, it will be hard to elute more strongly held materials.