Analysis of Calcium Ion:

Calcium ion within biological systems is determined fluorimetrically either through titration along with EDTA using calcein as indicator or through direct estimation using calcein as the reagent. The second method is based on complex creation among calcein and calcium ions in alkaline medium. The fluorophore so created has an excitation wavelength of 450 nm and emission is at 520 nm. Other cations are found to be noninterfering.

Various interesting biochemical analysis are completed through join the chromatographic separation and fluorimetry. The chromatographic methods coupled along with fluorimetry use a suitably developing fluorophore. An HPLC separation at room temperature is preferred. For instance, a variety of indols within biological fluids are analysed through fluorimetry.  The excitation is at 292 nm and emission occurs at 330 nm. Table provides a list of significant organic molecules present in biological samples and their fluorescence characteristics.

Table:  The fluorescence characteristics of organic molecules of biochemical origin