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Calcium imaging
This makes visible how Ca2+ signals spread in time and space by cells. Fluorescent dyes are used which on binding Ca2+ absorb UV light at a various wavelength than they do in the unbound state. The Neurons are preloaded with the dye and the emission of UV from the dye is observed in response to its excitation through the two distinct absorption wave- lengths. This provides a quantitative measure of how the concentration of Ca2+ changes in the neuron in real time.
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