Confocal Scanning Microscopy

Confocal scanning microscopy is a refinement of usual fluorescence microscopy which generates clearer images  of entire cells or larger specimens. the   fluorescent light emitted   by   the compound comes from molecules above and below the plane of focus, blurring the image and make it hard to determine the real three-dimensional molecular arrangement In   typical   fluorescence   microscopy. Only molecules in the plane of focus fluoresce with the confocal scanning microscope due to the use of a focused laser beam at the exciting wavelength. Laser beam is moved to different parts of the specimen, permitting a series of images to be taken at various depths through the sample. To provide the complete three- dimensional image the images are then combined by a computer. Deconvolution microscopy achieves the similar image sharpening effect as confocal scanning microscopy but through a various process.