Transcription in Eukaryotes:

The Eukaryotic transcription is more complex than prokaryotic transcription. For example, in eukaryotes the genetic material DNA and thus transcription is basically localized to the nucleus, where it is divided from the cytoplasm in which translation occurs through the entaamoeboic nuclear membrane. DNA is also available in mitochondria in the cytoplasm and mitochondria utilize a specialized RNA polymerase for transcription. This permits for the temporal regulation of gene expression by the sequestration of the RNA in the nucleus and permits for selective transport of RNAs to the cytoplasm where the ribosomes reside.

The basal eukaryotic transcription complex involves the RNA polymerase and additional proteins which are must for correct elongation and initiation. Typically, eukaryotes have several more genes than do prokaryotes, sometimes through various orders of magnitude. The genes of advanced organisms also tend to be huger owing to the facts that cis-acting sequences on the DNA can be located tens of thousands of base pairs away from the transcription begin site and in which a battery of regulatory factors is sometimes required to bring about proper regulation of certain genes.