Enzyme Kinetics

Enzyme kinetics is the learning of the chemical reactions which are catalysed via enzymes. In enzyme kinetics the reaction rate is measured and the effects of varying the conditions of the reaction investigated. Through Studying an enzyme's kinetics in this way can reveal the catalytic mechanism of this enzyme, its role in metabolism, how a drug or an agonist might inhibit the enzyme and how its activity is controlled.

Enzymes are commonly protein molecules which manipulate other molecules the enzymes' substrates. These goals molecules connect to an enzyme's active site and are transformed into products by a sequence of steps which is known as the enzymatic mechanism. These mechanisms can be seperated into multiple-substrate and single-substrate mechanisms. Kinetic studies on enzymes which only bind one substrate, like as triosephosphate isomerase, target to measure the affinity with that the enzyme binds this substrate and the turnover rate. When enzymes bind multiple substrates like as dihydrofolate reductase, enzyme kinetics can also described the sequence in that these substrates bind and the series in those products are released.