Advantages of HPLC technique:

In HPLC, the quantitative measurements are readily carried out on the chromatogram since the amount of substance present is  proportional to its peak area. The analysis of standard samples is often necessary for comparative purposes. The advantages of HPLC technique are as follows:

a) Non-volatile materials can be handled.

b) Ability to operate long columns at near optimum solvent velocity throughout their length.

c) The nature of mobile phases can be varied to produce either stepwise or gradient elution or both.