Applications of Atomic Fluorescence Spectrometry:

The atomic fluorescence spectrometry has been used within the analysis of more than about sixty various elements present in diverse variety of samples. The common use of AFS is to determine the concentration of elements within samples that is., elemental analysis. Within favourable conditions for a few elements (e.g. Cd, Pb, Tl), detection limits in the range of attogram (10^-18 g) have been obtained. The quantitative analysis using AFS could be carried out through employing standard calibration plot method or standard further method as in the case of other spectrometric methods. The linear calibration curves extend from 4 to 7 sequences of magnitude.

In a classic quantitative determination using a high intensity hollow cathode lamp of the element to be denotes as the radiation source, a series of solutions of the metal ions of varying concentration are prepared and aspirated within the flame and the corresponding fluorescence intensity is measured. A plot of fluorescence intensity versus concentration of the metal ion is plotted. That curve is linear at low concentration of metal ions, but is convex at higher concentration. The fluorescence intensity for the solution holding the analyte in unknown concentration is measured and the concentration of the metal ion is denoted from the standard curve.