Typical Applications Assignment Help

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Typical Applications:

The HPLC is the most successful technique in separating compounds as diverse as aminoacids, nucleic acids, and proteins in physiological samples, active drugs, steroids, carbohydrates, vitamins, dyestuffs, pesticides, polymers etc. It is used routinely for the assay of pharmaceutical products, the monitoring of drugs and metabolites in body fluids and for other biomedical, biochemical and forensic applications such as the detection of drugs of abuse. The determination of additives in foodstuffs and beverages including sugars, vitamins, flavorings and colorings and the quality control of polymers, plastics and resins are further examples of the wide and growing scope of HPLC. A summary of typical applications in various fields are listed in Table.

Table:  Typical Applications of HPLC Methods to Various Fields

Sl. No.

Field

Typical mixtures

1.

2.

3.

 

 

4.

5.

6.

7.

8.

 

 

9.

Pharmaceuticals Biochemicals Food products

 

Industrial chemicals Clinical medicines Polymers

Forensic chemistry

Pollutants

 

 

Quality control

Antibiotics, sedatives, steroids, analgesics

Aminoacids, proteins, carbohydrates,  lipids

Artificial sweetners, antioxidants, aflatoxins, additives

Condensed aromatics, dyes, surfactants, propellants. Bile acids, drug metabolites, urine extracts, estrogens Molecular weight determination and distribution. Drugs, poisons, blood alcohol, narcotics

Pesticides, herbicides, PCBs, phenols in environmental  samples

Purification from mixrure

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