Size exclusion:

The size exclusion chromatography or gel permeation chromatography is the most suited technique for the solutes with molecular weights 2000 or more and is also useful for preliminary investigation of unknown samples. It can be used for the determination of relative mass distribution for components of biochemical and polymer systems with an accuracy of 10%. Desalting is commonly employed to isolate the macromolecules from biochemical materials, where both simple and macromolecules may be present in an electrolyte solution. Dilute solutions of macro- molecules can be concentrated and isolated by adding dry gel beads to absorb the solvent relative molecular mass solutes.