Drug Abuse:

The HPLC is being widely used for the detection of drug abuse especially for the steroids by the athletes at the International games. A typical case of drug abuse is illustrated in Figure where oxphenbutazone, phenylbutazone and furosemide were detected in horse plasma.

The sample is filtered through 2 µ m membrane prior to direct injection into a 15 cm × 4.6 mm with 5 micron ISRP packing. A ternary mobile phase of isopropanol, tetrahydrofuran along with potassium hydrogen phosphate buffer was used at a flow rate of 1 mL/min.