Chiral Separation of Enantiomers:

A typical example of the separation of enantiomers of benzodiazepine Temazepam drug is illustrated in Figure where immobilized human serum albumin (HSA) bonded to silica was used as a reverse phase material and phosphate buffer-acetonitrile as mobile phase. A number of manufacturers now supply these columns specifically tailored for particular application.

Figure: Chiral separation of enantiomers of the benzodisazepine Temazepam drug showing separate peaks corresponding to d and l forms