Analytical Applications:

It may be important to point out that within analytical applications, there are further sub-divisions as follows:

Analytical group separations

One of the very common uses of gel filtration chromatography is the removal of interferences before the final determination. It is particularly important to remove low molecular mass interferences before the determination of macromolecular component in the mixture. The determination of protein in spinal fluid is a typical instance. The low molecular weight interferences are erased for the spectrophotometric determination of protein.

On a similar line, it is desirable to determine polysaccharides like insulin, amylose or dextran from low molecular mass sugar. Gel filtration chromatography has been used for the purpose, say, the removal of glucose from the mixture. A technique can also be used for the removal of high molecular weight interferences but this has not been used much.