Fluorescence Analysis Methods:

The applications of fluorimetry and phosphorimetry to the physical and life sciences have evolved quickly during the past decade. The increased interest within these appears to be because of advances in time resolution, methods of data analysis, and improved instrumentation, etc. Further, the advances in laser and detector technology have also contributed towards the renewed interest within fluorescence measurements in clinical and analytical chemistry. The key features of fluorescence spectrometry are its high sensitivity. It might achieve detection limits of various orders of magnitude lower than most of the other methods. Because of the low detection limits, fluorescence is hugely used for quantification of trace constituents of biological and environmental samples. Before taking up the applications of fluorescence within details it is worthwhile to learn about the primary fluorescence analysis methods. There are two kinds of fluorescence analysis methods that are direct analysis methods and indirect analysis methods as elaborates in the following sub-sections.