Quantitative Applications:

As discussed above, UV-VIS spectrometry has limited applications in qualitative analysis of the analytes; therefore, it is probably the most meaningful tool available for quantitative determinations in diverse areas. It is due its versatility, accuracy and sensitivity. It could be used for direct determination of a large number of organic, inorganic and biochemical species accurately at fairly low concentrations; viz., 10^-4 to 10^-5 or even lower. In addition to these, the convenience of conducting the determination and its reasonable selectivity make it a method of selection for quantitative determinations.

The other significant feature of this technique is which it might be used for the quantitative determination of analyte that do not absorb in the UV-VIS region. It is achieved through making them react along with a reagent that provides a product that absorbs in the region. The similar method could be used to undertake quantitative determination of all such species that have an absorption in the region but are present in system that holds other constituents that also absorb in the similar region. Therefore, absorbance measurements in visible or in UV region, are used within diverse areas. A few of the general ones are associated to the following quantitative aspects of solution chemistry.

1.         Analytical determination of metals and non-metals

2.         Analytical determination of organic compounds

3.         Determination of dissociation constants of organic acids and dyes

4.         Determination of metal-ligand formation constants

5.         Determination of kinetic stability of complexes