Methodology of quantitative determinations:

The primary for the first two aspects of quantitative analysis lies within the Beer and Lambert's law elaborates earlier. It includes comparing the extent of absorption of radiant energy at a particular wavelength through a solution of the test material along with a series of standard solutions. In the previous times, visual comparators were used for such determinations that were carried out with natural light (white light) and employed human eye to judge the color intensity. Within such an approach, a series of standard solutions of the analyte to be determined were prepared within tubes of constant depth and diameter. The sample solution was changed to a duplicate tube and diluted to the extent in which its color matched with one of the standard solutions. The concentration of the test solution was determined from the concentration of the standard solution through accounting for the dilution factor. The rest three aspects of solution chemistry need measurements of the UV-visible spectrum of the analyte as a function of pH or ligand concentration etc. Presently, a given analytical determination could be made in a number of probable ways. These techniques depend on the nature of the species being determined and follow types of methodology. Let us learn about the methodology of quantitative determinations.