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In Drosophila, the anterior determinant Bicoid is known to activate expression of the gene hunchback in the anterior half of the embryo. (Bicoid is a transcription factor, which binds to the promoter of the hunchback gene and stimulates transcription.) The gene chapeau is also activated by Bicoid, but requires higher levels of Bicoid to activate its expression than the hunchback gene requires. Where would you predict chapeau mRNA would be observed in a wild-type embryo?
a) Throughout the embryo
b) In the anterior half of the embryo
c) In the anterior quarter of the embryo
d) In the anterior ¾ of the embryo
e) In the posterior half of the embryo
f) In the posterior quarter of the embryo
Genomic libraries are screened by hybridization with a DNA probe which is complementary to component of the nucleotide sequence of the desired gene. The problem should be
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