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In Drosophila, the anterior determinant Bicoid is known to activate expression of the gene hunchback in the anterior half of the embryo. (Bicoid is a transcription factor, which binds to the promoter of the hunchback gene and stimulates transcription.) The gene chapeau is also activated by Bicoid, but requires higher levels of Bicoid to activate its expression than the hunchback gene requires. Where would you predict chapeau mRNA would be observed in a wild-type embryo?
a) Throughout the embryo
b) In the anterior half of the embryo
c) In the anterior quarter of the embryo
d) In the anterior ¾ of the embryo
e) In the posterior half of the embryo
f) In the posterior quarter of the embryo
In an experiment, it was necessary that you determine the concentration of dsDNA by using a spectrophotometer. You used 6 microliters of your purified dsDNA, and the reading at OD=
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