Why is boiling used as a control, Biology

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Substance A is being investigated to see if it is an enzyme. When substance A is mixed with substance B a reaction takes place. A control experiment is conducted using a sample of A which has been boiled.

   (a) Why is boiling used as a control?

   (b) If the reaction still worked after A had been boiled, what might be your interpretation?

 

(a) Boiling denatures enzymes. If a substance still works after boiling, it cannot be an enzyme.

(b) If the reaction still worked after A had been boiled, either A is not an enzyme or, if it is, it is not  necessary for the reaction.

 


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