Why capsulate staining is difficult, Biology

Assignment Help:

Why Capsulate Staining is Difficult?

Capsule staining is difficult because capsular material is soluble in water and may be dislodged or removed during vigorous washing. Negative staining can be used to visualize the capsule. Here presence of capsule is shown by mixing the bacteria in a loopful solution containing tiny colloidal suspension of coloured particles e.g. India Ink or nigrosin which cannot penetrate the cell or its capsule. After spreading the mixture in a thin film and air-drying, counter staining of cells is done with simple stains such as safranin, crystal violet etc. Capsule does not accept these dyes so appear as a halo surrounding coloured cells in a dark blue-black background.

 


Related Discussions:- Why capsulate staining is difficult

What is the first polar body, Q. What is the first polar body? How differen...

Q. What is the first polar body? How different is it from the oocyte II? In oogenesis the oogonium differentiates into oocyte I (2n) and this cell enters meiosis and after fini

How do cells get energy for their functioning, Q How do cells get energy fo...

Q How do cells get energy for their functioning? Cells get energy for their metabolic reactions from the breaking of organic molecules with high energetic content. This energy

Pollinia and massulae, Pollinia and Massulae In most cases the pollen ...

Pollinia and Massulae In most cases the pollen grains of each tetrad become separated from one another and they lie freely in the pollen sac. In some plants, mostly members of

What is the monohybridism, What is the monohybridism? The Monohybridism...

What is the monohybridism? The Monohybridism is the study of only one characteristic in the crossing of two pure individuals (hybridization) for that characteristic.

Moss stage - xerarch, Moss Stage - Xerarch The accumulation of soil, p...

Moss Stage - Xerarch The accumulation of soil, particularly in the crevices and depressions of rock favours the growth of certain xerophytic mosses, e.g., species of Polytrich

Copper - mineral elements, COPPER It is a trace element which is availa...

COPPER It is a trace element which is available in most of the fruits. Maximum in heart, brain, kidney & crustaceans. By its deficiency monkin's disease is caused. Cop

State the series of stimuli, State the series of stimuli ERPs a series ...

State the series of stimuli ERPs a series of stimuli such as tones or light flashes are presented to the participant, and the raw EEG for a precise one or two second period fol

Regulation of glycogenesis, Regulation  of Glycogenesis Glycogen syntha...

Regulation  of Glycogenesis Glycogen synthase,  the key enzyme in glycogenesis, is activated by insulin and glucose and inhibited by CAMP.

Pedigree charts, i need help with to do a pedigree charts.

i need help with to do a pedigree charts.

Define secondary level care - public nutrition, Define Secondary level care...

Define Secondary level care - public nutrition? More complex health problems of the community are resolved at the secondary level care through the district hospitals and the Co

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd