Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Why Ascending Method of Paper Chromatography is Preferred?
The ascending method is preferred because of the simplicity of the set up. In actual method the substance to be separated is applied as a spot in a suitable solvent with the help of a capillary tube and hot blower. The paper is then suspended vertically inside a sealed chamber to make its lower end dip into a solvent. Due to capillary action, the solvent ascends along the filter paper that forms the mobile phase, while the water held immobile by the cellulose fibres of the paper forms the stationary phase.
After the solvent front has run almost to the upper end of the filter paper, the latter is removed from the chamber and dried. The paper is then suitably stained to locate separate functions as coloured spots. Sometimes, to enhance the resolution of the spots and to verify the authenticity of the material, the chromatogram is subjected to two dimensional paper chromatography. After it is run in single dimension in one solvent system, it is dried and rotated to 900 and rechromatographed using a second altogether different solvent system. Finally the filter paper is dried and stained. The spots are better resolved after chromatography in the second dimension. In descending paper chromatography, the filter paper is suspended vertically down from the solvent chamber at the top so that the liquid and the solutes migrate down the paper.
CHEMICA L PROPERTIES Monosaccharides have two special chemical properties (i) Reducing Nature. All the monosaccharides are reducing sugars. It can reduce Cu2
summarise the comparative anatomy of the digestive system of the invertebrate and the vertebrate.
What are the factors responsible for corneal hydration? The factors responsible for corneal hydration are as follows: i. Stromal swelling pressure ii. Barrier function of
MICROFILAMENTS Discovered by Pelvitz. These are smallest cell structure. These are non-living structures. These are solid structures, consists of actin protein (c
Q. Show Acute Complications of Diabetes? Acute complications of diabetes include: Hypoglycemia or low blood sugar: The most frequent cause of low blood sugar is poor timing
Define Reagents for seliwanoff's test? - Sugar solutions of glucose, fructose, galactose, lactose, maltose, sucrose and starch - Resorcinol in dilute hydrochloric acid (1:
What is the neurotransmitter of the neuromuscular junction? How does the nervous system trigger muscle contraction? The nervous cells that trigger the muscle contraction are th
Find the initial osmotic pressure at room temperature for a cell if the only ions present are KCl and NaCl on either side of the membrane. Assume the concentrations for K+ and Na+
Mechanical requirements of implant materials Dental implant s must be able to sustain and transfer loads -It should have adequate mechanical strength in order to distribute th
A complete gut is sometimes called tube within a tube. Why?
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd