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A restriction enzyme or restriction endonuclease is an enzyme which cuts DNA at particular recognition nucleotide sequences with Type II restriction enzymes cutting double-stranded DNA known as restriction sites. Like enzymes, make in archaea and bacteria, are thought to have evolved to give a defense mechanism against occupy viruses. Within a bacterial host the restriction enzymes selectively cut up foreign DNA in a procedure called as restriction; host DNA is methylated by a modification enzyme (a methylase) to protect it from the restriction enzyme's activity. Cooperatively, these two procedures form the restriction modification system. For cut the DNA a restriction enzyme create two incisions, once by every sugar-phosphate backbone of the DNA double helix.
You now want to express the gene in E coli, but unfortunately it has an intron in it that will not be processed out in bacteria, hence it must be removed. (a) Design P
NON-PROTEI N AMINO ACIDS Non-protein amino acids are the ones which are not incorporated in proteins but occur freely or in combined state in non-protein substances. Their
Define Water Distribution and Compartments of Body Water? Each one of us has a veritable 'sea within', held in place by multiple membranes and our protective envelope of skin.
Define methods of Food Preservation? Food preservation is one of the oldest technologies used by human beings. The perishable food materials like fruits, vegetables, milk, meat
Limitations of Ecological Pyramids The pyramid of energy is a significant improvement over the previous two types of ecological pyramids, yet all of them overlook one or anot
Explain Implant Integrity Implant Integrity: A horizontal dark line at the abutment level is most probably due to screw-loosening and separation of elements. This may happen
What is gene? Gene is a sequence of DNA nucleotides that codifies the production of a protein.
Sexually-transmitted diseases Many travelers have new sexual contacts during their journeys and are at increased risk for acquiring sexually transmitted diseases including HIV
A genomic DNA library is made from the genomic DNA of an organism. For instance, a mouse genomic library could be made by digesting mouse nuclear DNA with a restriction nuclease to
Full - thickness flap with a c-shaped excisional incision In case where there is adequate soft tissue, both in terms of papillary height and labial bulk, the excision of the ep
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