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Consider a simple spherical model cell that consists of cytoplasm and a plasma membrane.
The cell's initial volume is 2 nL and contains 0.2 M protein. The cell is placed in a large volume of 0.05 M CaCl2. Neither Ca++ nor Cl- can cross the plasma membrane and enter the cell. Are the 0.05 M CaCl2 solutions hypotonic, isotonic or hypertonic relative to the osmolarity inside the cell? Describe what happens to the mathematically cell as it achieves equilibrium in this new environment. What will be the final osmolarity of the cell? What will be its final volume?
Determination Reductive hydrolysis of folic acid produces 4-aminobenzoyl glutamic acid which is determined photometrically after diazotization and coupling with N-(1-naphthyl)
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Explain the Sponge Method? In the sponge method, sterilized sponge with 45 x 5 cm contact surface and free from antimicrobial agent is used. Aseptically, it is moistened with 1
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